ATE142272T1 - Nukleinsäure-amplifikation unter verwendung eines einzelprimers - Google Patents
Nukleinsäure-amplifikation unter verwendung eines einzelprimersInfo
- Publication number
- ATE142272T1 ATE142272T1 AT90300528T AT90300528T ATE142272T1 AT E142272 T1 ATE142272 T1 AT E142272T1 AT 90300528 T AT90300528 T AT 90300528T AT 90300528 T AT90300528 T AT 90300528T AT E142272 T1 ATE142272 T1 AT E142272T1
- Authority
- AT
- Austria
- Prior art keywords
- polynucleotide
- single stranded
- sequence
- analyte
- stranded polynucleotide
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 abstract 15
- 108091033319 polynucleotide Proteins 0.000 abstract 15
- 239000002157 polynucleotide Substances 0.000 abstract 15
- 239000012491 analyte Substances 0.000 abstract 5
- 230000000295 complement effect Effects 0.000 abstract 2
- 230000001419 dependent effect Effects 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- -1 nucleoside triphosphates Chemical class 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- 235000011178 triphosphate Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29928289A | 1989-01-19 | 1989-01-19 | |
| US39979589A | 1989-08-29 | 1989-08-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE142272T1 true ATE142272T1 (de) | 1996-09-15 |
Family
ID=26971136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT90300528T ATE142272T1 (de) | 1989-01-19 | 1990-01-18 | Nukleinsäure-amplifikation unter verwendung eines einzelprimers |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US5827649A (de) |
| EP (1) | EP0379369B1 (de) |
| JP (1) | JP3109810B2 (de) |
| AT (1) | ATE142272T1 (de) |
| CA (1) | CA2008096A1 (de) |
| DE (1) | DE69028325T2 (de) |
| ES (1) | ES2093633T3 (de) |
Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5595891A (en) * | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
| US5439793A (en) * | 1990-07-19 | 1995-08-08 | Syntex (U.S.A.) Inc. | Method for producing a polynucleotide having an intramolecularly base-paired structure |
| CA2049879A1 (en) * | 1990-08-30 | 1992-03-01 | Keith C. Backman | Controlled initial target-dependent production of templates for ligase chain reaction |
| CA2046713A1 (en) * | 1990-10-16 | 1992-04-17 | Richard M. Martinelli | Amplification of midivariant dna templates |
| WO1992020820A1 (en) * | 1991-05-15 | 1992-11-26 | Vanderbilt University | Method to determine metastatic potential of tumor cells |
| ES2112302T3 (es) | 1991-10-11 | 1998-04-01 | Behringwerke Ag | Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos. |
| AU658962B2 (en) * | 1991-11-15 | 1995-05-04 | Igen, Inc. | Rapid assays for amplification products |
| ZA929319B (en) * | 1991-12-11 | 1993-05-24 | Igen Inc | Method for exponential amplification of nucleic acid by a single unpaired primer. |
| EP0675966B1 (de) * | 1992-02-19 | 2004-10-06 | The Public Health Research Institute Of The City Of New York, Inc. | Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren |
| ATE152180T1 (de) * | 1992-07-31 | 1997-05-15 | Behringwerke Ag | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
| FR2703053B1 (fr) * | 1993-03-26 | 1995-06-16 | Genset Sa | Oligonucleotides agrafes et semi-agrafes, procede de preparation et applications . |
| US6294323B1 (en) * | 1993-04-14 | 2001-09-25 | Behringwerke Ag | Self initiating single primer amplification of nucleic acids |
| US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| CA2231184A1 (en) * | 1995-09-20 | 1997-03-27 | George Tice Jr. | Control for pcr |
| JP2000500976A (ja) * | 1995-11-25 | 2000-02-02 | ベーリンガー マンハイム ゲーエムベーハー | 多数の二本鎖核酸の生成方法 |
| ES2270467T3 (es) * | 1996-07-16 | 2007-04-01 | Gen-Probe Incorporated | Metodos para detectar y amplificar secuencias de acido nucleico utilizando oligonucleotidos modificados que tienen una tm especifica de la diana mas elevada. |
| US7070925B1 (en) * | 1996-07-16 | 2006-07-04 | Gen-Probe Incorporated | Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe |
| US6326143B1 (en) | 1996-11-22 | 2001-12-04 | Roche Diagnostics Gmbh | Method for generating multiple double stranded nucleic acids |
| US6025133A (en) * | 1996-12-30 | 2000-02-15 | Gen-Probe Incorporated | Promoter-sequestered oligonucleoside and method of use |
| JP4646404B2 (ja) | 1998-07-02 | 2011-03-09 | ジェン−プローブ・インコーポレーテッド | 分子トーチ（ｔｏｒｃｈ） |
| WO2000004193A1 (en) * | 1998-07-20 | 2000-01-27 | Yale University | Method for detecting nucleic acids using target-mediated ligation of bipartite primers |
| US6703228B1 (en) * | 1998-09-25 | 2004-03-09 | Massachusetts Institute Of Technology | Methods and products related to genotyping and DNA analysis |
| EP1001037A3 (de) * | 1998-09-28 | 2003-10-01 | Whitehead Institute For Biomedical Research | Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus |
| CA2345441A1 (en) | 1998-10-27 | 2000-05-04 | Affymetrix, Inc. | Complexity management and analysis of genomic dna |
| US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
| US6958225B2 (en) | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| AU2002232538A1 (en) * | 2000-12-19 | 2002-07-01 | Discoverx | Strand displacement detection of target nucleic acid |
| US6872529B2 (en) | 2001-07-25 | 2005-03-29 | Affymetrix, Inc. | Complexity management of genomic DNA |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US6977153B2 (en) | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| BRPI0408967B8 (pt) | 2003-03-31 | 2021-07-27 | Hoffmann La Roche | kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas |
| CN1280428C (zh) * | 2003-05-19 | 2006-10-18 | 清华大学 | 一种基于微小颗粒的生物芯片系统及其应用 |
| CN1950519A (zh) * | 2004-02-27 | 2007-04-18 | 哈佛大学的校长及成员们 | 聚合酶群落荧光原位测序珠子 |
| JP4728663B2 (ja) * | 2004-03-22 | 2011-07-20 | シスメックス株式会社 | 標的物質検出用プローブセット及び標的物質検出方法 |
| JP2005304489A (ja) * | 2004-03-24 | 2005-11-04 | Sysmex Corp | 標的物質検出用プローブセット及び標的物質検出方法。 |
| US7939251B2 (en) | 2004-05-06 | 2011-05-10 | Roche Molecular Systems, Inc. | SENP1 as a marker for cancer |
| CA2572472C (en) * | 2004-07-01 | 2013-08-13 | Gen-Probe Incorporated | Methods and compositions to detect nucleic acids in a biological sample |
| EP1623996A1 (de) * | 2004-08-06 | 2006-02-08 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek |
| US8309303B2 (en) | 2005-04-01 | 2012-11-13 | Qiagen Gmbh | Reverse transcription and amplification of RNA with simultaneous degradation of DNA |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| US9044715B2 (en) * | 2007-08-22 | 2015-06-02 | Colorado School Of Mines | Unsupported palladium alloy membranes and methods of making same |
| US9388457B2 (en) | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
| US9074244B2 (en) | 2008-03-11 | 2015-07-07 | Affymetrix, Inc. | Array-based translocation and rearrangement assays |
| CA2723726C (en) | 2008-05-13 | 2017-09-12 | Michael M. Becker | Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences |
| AU2009324884B2 (en) * | 2008-11-25 | 2013-10-03 | Gen-Probe Incorporated | Compositions and methods for detecting small RNAs, and uses thereof |
| US20110059453A1 (en) * | 2009-08-23 | 2011-03-10 | Affymetrix, Inc. | Poly(A) Tail Length Measurement by PCR |
| WO2012048340A2 (en) | 2010-10-08 | 2012-04-12 | President And Fellows Of Harvard College | High-throughput immune sequencing |
| EP3561159B1 (de) | 2010-10-08 | 2023-09-20 | President and Fellows of Harvard College | Einzelzellen-barcoding mit hohem durchsatz |
| EP3604555B1 (de) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequenzierung durch strukturanordnung |
| US10202628B2 (en) | 2012-02-17 | 2019-02-12 | President And Fellows Of Harvard College | Assembly of nucleic acid sequences in emulsions |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| EP2875458A2 (de) | 2012-07-19 | 2015-05-27 | President and Fellows of Harvard College | Verfahren zur speicherung von informationen mithilfe von nukleinsäuren |
| US9476089B2 (en) | 2012-10-18 | 2016-10-25 | President And Fellows Of Harvard College | Methods of making oligonucleotide probes |
| EP2971184B1 (de) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Verfahren zur erzeugung einer dreidimensionalen nukleinsäure mit matrix |
| WO2015127242A1 (en) | 2014-02-21 | 2015-08-27 | President And Fellows Of Harvard College | De novo design of allosteric proteins |
| ES2891738T3 (es) | 2014-04-09 | 2022-01-31 | Scripps Research Inst | Incorporación de nucleósidos trifosfato no naturales o modificados a células a través de transportadores de ácido nucleico trifosfato |
| US11198855B2 (en) | 2014-11-13 | 2021-12-14 | The Board Of Trustees Of The University Of Illinois | Bio-engineered hyper-functional “super” helicases |
| WO2018213803A1 (en) | 2017-05-19 | 2018-11-22 | Neon Therapeutics, Inc. | Immunogenic neoantigen identification |
| SG11202000167SA (en) | 2017-07-11 | 2020-02-27 | Synthorx Inc | Incorporation of unnatural nucleotides and methods thereof |
| US11622993B2 (en) | 2017-08-03 | 2023-04-11 | Synthorx, Inc. | Cytokine conjugates for the treatment of autoimmune diseases |
| EP3613855A1 (de) | 2018-08-23 | 2020-02-26 | Clariant Produkte (Deutschland) GmbH | Verfahren zur herstellung einer nukleinsäurenbibliothek |
| CA3127689A1 (en) | 2019-02-06 | 2020-08-13 | Synthorx, Inc. | Il-2 conjugates and methods of use thereof |
| EP4555102A1 (de) | 2022-07-12 | 2025-05-21 | Topogene Inc. | Skalierbare replikation von dna-arrays mit submikron-auflösung |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US888058A (en) * | 1907-09-25 | 1908-05-19 | Charles Berg | Shrubbery-cutter. |
| US4777129A (en) * | 1983-12-12 | 1988-10-11 | Molecular Diagnostics, Inc. | Nucleic acid probe detectable by specific nucleic acid binding protein |
| US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4882269A (en) * | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
| EP0286642A4 (de) * | 1985-12-17 | 1990-06-27 | Genetics Inst | Testverfahren mittels polynukleotiden versetzung und reagenzkomplex. |
| GB8612087D0 (en) * | 1986-05-19 | 1986-06-25 | Ici Plc | Hybridisation probes |
| US4908307A (en) * | 1986-12-19 | 1990-03-13 | Karin D. Rodland | Hybridization method and probe for detecting nucleic acid sequences |
| AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| GB8721072D0 (en) * | 1987-09-08 | 1987-10-14 | Volex Group Plc | Cable dielectric |
| US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| US5508178A (en) * | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
-
1990
- 1990-01-18 AT AT90300528T patent/ATE142272T1/de not_active IP Right Cessation
- 1990-01-18 ES ES90300528T patent/ES2093633T3/es not_active Expired - Lifetime
- 1990-01-18 CA CA002008096A patent/CA2008096A1/en not_active Abandoned
- 1990-01-18 DE DE69028325T patent/DE69028325T2/de not_active Expired - Fee Related
- 1990-01-18 EP EP90300528A patent/EP0379369B1/de not_active Expired - Lifetime
- 1990-01-19 JP JP02010457A patent/JP3109810B2/ja not_active Expired - Fee Related
-
1994
- 1994-05-16 US US08/242,931 patent/US5827649A/en not_active Expired - Fee Related
-
1995
- 1995-05-09 US US08/438,149 patent/US6124090A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0379369A3 (de) | 1991-07-03 |
| EP0379369B1 (de) | 1996-09-04 |
| JP3109810B2 (ja) | 2000-11-20 |
| US5827649A (en) | 1998-10-27 |
| US6124090A (en) | 2000-09-26 |
| CA2008096A1 (en) | 1990-07-19 |
| DE69028325D1 (de) | 1996-10-10 |
| ES2093633T3 (es) | 1997-01-01 |
| JPH02268683A (ja) | 1990-11-02 |
| EP0379369A2 (de) | 1990-07-25 |
| DE69028325T2 (de) | 1997-04-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |