WO2004039831A3 - Peptide nucleic acid probes for analysis of enterococcus faecium - Google Patents

Peptide nucleic acid probes for analysis of enterococcus faecium Download PDF

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Publication number
WO2004039831A3
WO2004039831A3 PCT/US2003/034350 US0334350W WO2004039831A3 WO 2004039831 A3 WO2004039831 A3 WO 2004039831A3 US 0334350 W US0334350 W US 0334350W WO 2004039831 A3 WO2004039831 A3 WO 2004039831A3
Authority
WO
WIPO (PCT)
Prior art keywords
enterococcus faecium
analysis
nucleic acid
peptide nucleic
acid probes
Prior art date
Application number
PCT/US2003/034350
Other languages
French (fr)
Other versions
WO2004039831A2 (en
Inventor
Henrik Stender
Original Assignee
Advandx, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advandx, Inc. filed Critical Advandx, Inc.
Priority to AU2003286759A priority Critical patent/AU2003286759A1/en
Publication of WO2004039831A2 publication Critical patent/WO2004039831A2/en
Priority to US11/117,642 priority patent/US20050272078A1/en
Publication of WO2004039831A3 publication Critical patent/WO2004039831A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed are PNA based probes that include a nucleobase sequence suitable for the analysis of Enterococcus faecium. In one embodiment, the probe is complementary to a target sequence of Enterococcus faecium 23 rRNA or rDNA or its complement. The invention has a wide range of important applications including use to detect Enterococcus faecium in a biological sample.
PCT/US2003/034350 2002-10-28 2003-10-27 Peptide nucleic acid probes for analysis of enterococcus faecium WO2004039831A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003286759A AU2003286759A1 (en) 2002-10-28 2003-10-27 Peptide nucleic acid probes for analysis of enterococcus faecium
US11/117,642 US20050272078A1 (en) 2002-10-28 2005-04-27 Peptic nucleic acid probes for analysis of Enterococcus faecium

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42165702P 2002-10-28 2002-10-28
US60/421,657 2002-10-28

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/117,642 Continuation-In-Part US20050272078A1 (en) 2002-10-28 2005-04-27 Peptic nucleic acid probes for analysis of Enterococcus faecium

Publications (2)

Publication Number Publication Date
WO2004039831A2 WO2004039831A2 (en) 2004-05-13
WO2004039831A3 true WO2004039831A3 (en) 2011-11-03

Family

ID=32230245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/034350 WO2004039831A2 (en) 2002-10-28 2003-10-27 Peptide nucleic acid probes for analysis of enterococcus faecium

Country Status (3)

Country Link
US (1) US20050272078A1 (en)
AU (1) AU2003286759A1 (en)
WO (1) WO2004039831A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006252346B2 (en) * 2005-06-02 2012-06-28 Advandx, Inc. Peptide nucleic acid probes for analysis of microorganisms
ATE449190T1 (en) * 2006-03-25 2009-12-15 Univ Ruprecht Karls Heidelberg METHOD FOR MICROSCOPIC DETERMINING THE LOCATION OF A SELECTED, INTRACELLULAR DNA SECTION OF KNOWN NUCLEOTIDE SEQUENCE
DK2158334T3 (en) * 2007-05-18 2013-03-04 Advandx Inc DETECTION OF METHICILLIN-RESISTENT STAPHYLOCOCCUS
EP2022860B1 (en) * 2007-07-30 2011-09-21 Universität Ulm An oligonucleotide probe and a process for detecting or discriminating microorganisms in a sample
DK178306B1 (en) * 2013-06-16 2015-11-23 Stender Diagnostics V Henrik Stender Determination of Intracellular Bacteria

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEIMFOHR ET AL.: "In situ Identification of Lactococci", ENTEROCOCCI AND STRPTOCOCCI. SYSTEM. APPL. MICROBIOL., vol. 16, 1993, pages 450 - 456 *
GEIGER ET AL.: "PNA Array Technology In Molecular Diagnostics.", NUCLEOSIDES & NUCLEOTIDES, vol. 17, no. 9-11, 1998, pages 1717 - 1724 *
STENDER ET AL.: "PNA for rapid microbiology.", JOURNAL OF MICROBIOLOGICAL METHODS., vol. 48, 2002, pages 1 - 17, XP002390590, DOI: doi:10.1016/S0167-7012(01)00340-2 *

Also Published As

Publication number Publication date
WO2004039831A2 (en) 2004-05-13
AU2003286759A1 (en) 2004-05-25
US20050272078A1 (en) 2005-12-08
AU2003286759A8 (en) 2011-11-24

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